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The supernatant was collected (cytosolic fraction) and the pellet was suspended in a fractionation buffer mix (mitochondrial fraction).
Under stress conditions without UPS inhibition, however, the turnover of ubiquitinated Mfn1/2 in the cytosol is probably occurring too rapidly to be detected in a fractionation experiment.
In a fractionation study of human and rat brains, the highest enzymatic activity of Pegpep1 was found in the synaptosomal and myelinic fractions [35], and it was proposed that the enzyme may be involved in the inactivation of neuropeptides.
The pellet was suspended in a fractionation buffer mix containing DTT (1 mM) and homogenized in a Dounce tissue grinder.
The total recommended dose amounts to 54 Gy (1.8 Gy qd) for low-grade gliomas, and 60 Gy for GBM, in a fractionation of 5 × 2 Gy/week.
To isolate mitochondria, cells were lysed in a fractionation buffer (20 mM HEPES-HCl (pH 7.5), 10 mM KCl, 15 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 250 mM sucrose), homogenized and centrifuged for 10 min at 800 × g.
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In this way, it accounts not only for rearrangements which includes a whole interval in its scope but also for rearrangements which disrupt an interval, in that a fractionation involving such an interval will generally be automatically counted as two intervals, resulting in two virtual units instead of one.
The hope now, environmentalists say, is to halt work on this project in Paracas, at a fractionation plant that would separate gas liquids and pipe them into a marine terminal.
A common function of 14-3-3 14-3-3 14-3-3o traproteinsndisg partoers in trapcytosol [ 25], and we therefore more specitscally investigated the localization of exogenously expressed SIK2 in adipocytes, using a fractionation protocol.
The fractionation factor associated with storage of Mo, α3, has a similar effect on the model as α1, and in the absence of a fractionation during uptake will define the upper bound for fenz = 1.
We used two complementary methods to address this issue, i.e. an in situ approach and a fractionation of the colonic epithelial cells and subsequent analysis of Ki67-positive cells on cytospin preparations of the different fractions.
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