In a distinct set of experiments following neutrophil isolation, the apoptotic cells were removed from both WT and AnxA1−/− mice, using Annexin V+ve microbeads (Miltenyi Biotec Ltd. Surry, UK) per the manufacturer's instructions.
A total of 94 maize inbred lines were phenotyped in two distinct experiments (October 2007, May 2010) under greenhouse conditions using the same artificial inoculation procedure as RIL populations.
A scrambled sequence and a siRNA directed against the GAPDH gene were used as controls in three distinct experiments, each performed in duplicate.
The japonica panel was evaluated for blast disease resistance in two distinct experiments in 2014 and 2015.
Then, the effect of the sign_split function, the dx parameter and the force field used to assign partial charges were measured in three distinct experiments.
The indica panel was evaluated for blast disease resistance in two distinct experiments in 2011 and 2012 in the same breeding station located close to the village of Andranomanelatra, 130 km south of the town of Antananarivo in Madagascar (19°47′ S, 47°06′ E) at an altitude of 1635 m a.s.l.l
Analyses were performed in triplicate in three distinct experiments and mean results are presented.
Cowhage spicules were subsequently applied in the same fashion as for the control, in four distinct experiments.
Our results were all confirmed in triple distinct experiments, all provided of positive and negative cell controls.
ENCODE contains genomic binding information of 119 transcription-related factors in >450 distinct experiments providing regulatory information crucial for understanding basic principles of human biology and disease.
