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The key marker genes selected for defining a path's distance may be previously known genes (e.g. genes known to be involved in a differentiation process) or differentially expressed genes.
This process occurs via bone morphogenic proteins (BMPs) that induce runt-related transcription factor 2 (Runx2), leading to increased alkaline phosphatase activity. 1 Conversely, osteoclasts resorb bone matrix 3 and differentiate from the hematopoietic cell lineage upon stimulation in a differentiation process called osteoclastogenesis.
To investigate whether PASCs can be differentiated into hormone-producing cells, both PASC1 and PASC2 were cultured in a differentiation medium for 2 weeks, followed by stimulation of hypothalamic hormones, including GHRF, PRP, LHRH, TRH, and CRH.
The study resulted in a differentiation into two sedimentation phases.
Iron complexes would be less soluble at soil pH, resulting in a differentiation of an upper Fe-rich Bhs1 horizon and a lower Bhs2 Al-rich horizon.
For immunocytochemistry, the HSCs were cultivated in a differentiation medium and fixed and immunostained after 4 days with 4′,6-diamidino-2-phenylindole (DAPI) and (tetra-methyl rhodamine isothiocyanate -phalloidin (TRisothiocyanate -phalloidinsly [33].
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Since the expression level of HP1γ affects the ability of ESCs to differentiate, we wondered whether HP1γ might be regulated in a differentiation-dependent manner.
In the developing lens, which has no desmosomes, we discovered that vimentin became linked to N-cadherin complexes in a differentiation-state specific manner.
We recently demonstrated that adipocytes release DPP4 in a differentiation-dependent manner (5).
Analysis of Fcor mRNA and protein levels indicated that FCoR is expressed in 3T3-F442A cells in a differentiation-dependent manner.
In XBP-1KO/μS−/− B cells, we observed a massive increase of IRE-1 levels in a differentiation-dependent manner, despite the fact that secreted IgM is absent in these B cells.
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