In line with previous observations, the altered pattern of differentiation markers expressed in osteoblasts accompanied by a different staining pattern of mineralization and increased proliferation, suggests that LNCaP-19 cells stimulate an immature population of osteoblasts that contributes to the major part of the newly synthesized bone.
Moreover, we also noticed a different staining pattern in proximal tubules: in control rats, a strong signal was localized at the apical plasma membrane, whereas kidney sections of nephritic rats displayed a more diffuse staining throughout the tubular epithelial cells (Fig. 6D 6F).
The results from the TMA "prostatectomy 3" proved that this effect was also found when immunohistochemical staining was performed with a different antibody on a different staining automat, in a different laboratory, and evaluated by a different observer.
By comparison, previous studies demonstrated that HCC15.1 accumulates in a perinuclear location and results in a distinctively different staining pattern than the H1299.3 peptide.
The authors did report differences in OS between different staining patterns: a perinecrotic staining pattern, in which cells stain more strongly as the distance to the nearest blood vessel increases, was associated with worse OS (P < 0.01) and LRC (P = 0.01) when compared to diffuse or no expression of CA-IX.
Some clinical and laboratory features showed differences in proportions over different staining patterns (table 1).
To account for variations in staining intensity from images obtained in different staining experiments, the data were normalized to controls stained side-by-side.
In addition, different staining combinations confirmed that CD163pos DDC expressed CD16, CD206 and CD209, but neither CD207 nor CadM1 (data not shown).
NMO-Ig Abs resulted in different staining patterns when binding to full length AQP4 in contrast to the M-23 AQP4 isoform.
A simple diffusion model has been previously used to attempt to explain changes in iodine concentrations at a certain position in a mouse embryo given different staining durations [ 15].
