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So the present adsorption experiments were measured and repeated in a dark condition.
The rectification ratio is as high as 535 at −1 V (1695 at −2 V) in a dark condition.
The purified nanoparticles were finally suspended in distilled water and stored at 4 °C in a dark condition.
The dye solutions containing gel adsorbents were stirred slowly and continuously at room temperature in a dark condition.
The APTES film formation was carried out for 1 h at room temperature with stirring in a dark condition.
All tested samples were dissolved in PBS. 5 mL of fresh ABTS solution was mixed with 500 µL of tested samples for 2 h in a dark condition.
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To visualize oil bodies and assess their morphology, localization and numbers in the cultured cells Nile Red staining was applied (0.1 mg mL-1 in acetone in a 1 100 ratio) in dark conditions, using a similar procedure to that described for determination of the cells' neutral lipid contents.
The sample was allowed to react with 3 mL of the FRAP solution for 30 min in the dark condition [35] forming a colored complex.
After stirring the solution for 40 min in dark condition, only a little decrease in the absorption peak intensity is observed for RhB, which indicates a weak dye adsorption of the nanocomposite.
N, N-dimethylformamide (DMF) was added into the powder obtained from 1 g algal material to extract chlorophyll a in dark conditions at 4°C for 48 hr.
The seeds were incubated at 30 ± 2°C in dark condition in growth chamber.
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