Thus, in patients, the adjunction of a correct orientation and a warning tone enhanced their ability especially in difficult conditions e.g with incongruent stimuli.
To confirm integration of hph+trpC at the desired locus and in the correct orientation, a primer from within hph and within 200 bp of a flanking region used to guide insertion were used to amplify a diagnostic region of approximately 1.5 kb.
The simulated maps aligned with the axes, except for the Willshaw model which initially produced a map in the correct orientation [ Fig. 3(E ], but then drifted gradually over time [ Fig. 3(F)].
The overlap was considered to be in the correct orientation when a gene targeted by an upregulated microRNA was decreased in the gene expression profile, or when a gene targeted by a downregulated microRNA was increased in the gene expression profile.
The SVNeo cassette confers G418 resistance to cells that integrate and express the transposon vector, but the gene-trap tTA cassette can intercept splicing and express tTA only if the transposon lands in an expressed gene in the correct orientation.
Plasmids containing an insert in the correct orientation were sequence verified.
Integration of the transposon within an intron in the correct orientation results in the transcription and subsequent splicing of the trapping exon into the mature mRNA of the targeted gene.
Plasmids were checked for the presence and orientation of the insert by PCR, and those plasmids that contained an insert in the correct orientation were used to transform F62.
Reads mapped as part of a perfect pair (in the correct orientation and separated by the correct distance) and hang off a contig end are used to extend the contig.
For paired-end reads, two reads belonging to a pair were aligned together with both in the correct orientation and with a proper span size on the reference.
Reads that are mapped in the correct orientation (paired end reads orientated inwards, →←) and at the correct distance often termed proper pairs are coloured blue.
