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A mixture of 200 300 pmol of the two labelled samples was concentrated in a Concentrator Plus (Eppendorf™, Hamburg, Germany).
A mixture of 350 400 pmol of the two labeled samples was concentrated in a Concentrator Plus (Eppendorf™, Hamburg, Germany).
The eluted protein was buffer-exchanged into buffer C (0.1% DDM, 20 mM Tris, pH 7.5) and concentrated in a concentrator with a 100 kDa cut-off membrane (Millipore).
The SB hemicellulosic hydrolysate was vacuum concentrated at 70°C in a concentrator and further detoxified by sequential calcium oxide-activated charcoal pretreatment according to Alves et al. [ 52].
The QD pellet was dried in a Concentrator Plus centrifugal concentrator (Eppendorf, USA) for 2 min.
After vigorous shaking, the fungal material was pelleted, the supernatant discarded and the mycelium dried in a concentrator (room temperature, 60 min; Eppendorf, Hamburg, Germany).
Similar(39)
After that, 100 μl of the water/methanol phase was dried in a vacuum concentrator (Eppendorf Concentrator Plus) for derivatization [ 77] for GC/MS analysis.
Supernatants from two extraction steps were pooled and evaporated until dryness in a vacuum concentrator (CentriVap Centrifugal Concentrator, Labconco, Kansas City, MO, USA) at 30°C.
Protein extracts for pooled samples of eggs, larvae, and adult males were concentrated in a vacuum concentrator prior to loading into gels for electrophoresis.
The filtered extracts were combined and concentrated in a vacuum concentrator.
Eluted peptides were concentrated in a vacuum concentrator, and reconstituted with 20 µL of 0.1% trifluoroacetic acid.
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