Sequenced in 2009, a comprehensive annotation of the sorghum genome and the development of functional genomics resources is key to enable the discovery and deployment of regulatory and metabolic genes and gene networks for crop improvement.
In order to provide a comprehensive annotation of functional tRNA genes in the B. taurus genome we used a combination of approaches which involved tRNAscan-SE, RepeatMasker and comparative genomics to overcome the issue of SINEs.
Despite recent advances in computational ORFs prediction, a comprehensive annotation of protein-coding genes remains challenging, as fully automated annotation processes often lead to wrong prediction of protein functions [ 4], and therefore time-intensive manual curation is often essential.
An hybrid annotation approach, based on integration of gene predictions and massive parallel sequencing of transcripts, is thus needed to perform a comprehensive annotation of genes in a fungal genome.
However, additional data, including a comprehensive annotation of the enhancer landscape in normal endometrial cells and in endometrial cancers, are needed to further elucidate this important mechanism.
For the first time, a comprehensive annotation of regulatory RNAs in MAP was performed.
We present a comprehensive annotation of structured RNAs in the pig genome.
Here, we attempt to reconcile these differences by conducting a comprehensive annotation of the cathelicidin locus in Bos taurus using homology-based search methods of the BLAST family of programs [ 37] and the more sensitive Hidden Markov Models (HMM) approach [ 38].
As part of that landmark development we performed a comprehensive annotation of miRNA genes, now fully described in this report.
We provide a comprehensive annotation of canonical and non-canonical p53 REs in the human genome, ranked on predicted transactivation potential.
