The marvellous Missy Elliot based "Lose Control" from a "Clear" sample, as did T.I. in "Lay Me Down," feat.
Even though data results are from the largest sampling effort available, the lack of a clear sampling strategy in the collection of wild-bird data precludes a definite answer to whether poultry flocks were infected with HPAI (H5N1) from infected wild birds or vice versa.
This is reflected by a clearer sample separation in the PCA plot (Figure 7B).
To check the amplification efficiency, we selected 6 genes (3 reference genes and 3 target genes) and measured their efficiency in 4 CLEAR samples that were used in generating RA datasets in a dilution series.
Samples were dissolved at a concentration of 4 mg/ml in DMSO-LiBr by overnight rotation at room temperature, followed by 30 min heating in an oven at 80 °C obtaining clear sample solutions.
This method resulted in a clear ordering of the samples taken from our test-set (Figure 6).
In addition, a tree is comprehensible to a wide audience and results in a clear division of the original sample in groups of high and low risk making it useful to policy makers.
Unsupervised hierarchical clustering of the modulated probe sets confirmed clear sample segregation in the two phenotypic groups.
Samples associating in a clear cluster with a reference subspecies sequence were assigned to that subspecies, as described previously [107].
Briefly, for fresh samples stool in a clear transparent container was processed within 30 min of collection.
