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The association of F8-IL10 proteins with blood cells was studied in a centrifugation assay with radiolabeled protein.
The mononuclear fractions were enriched with MSCs, which were isolated in a centrifugation gradient using Lymphoprep (Atom, Barcelona, Spain).
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In fission yeast, a centrifugation method has been developed to misplace the nucleus away from the cell center.
Cell synchronization, which includes a centrifugation in a density gradient of silica (Ludox) at 4°C, was performed as previously described [ 13], using 1 L of culture.
After a centrifugation in the presence of 200 µl of 70% ethanol and two consecutive centrifugations in 90% ethanol, at 14 000 g for 30 s, purified DNA was eluted in 15 µl of Elution Buffer (CP8).
The spin column method was observed to routinely provide a total matrix reduction of >90% from typical wheat samples in a single centrifugation step.
For each strain 1 g of dried and milled fruit body was placed in a glass centrifugation tube and immersed in 30 mL 96% EtOH.
The preparation of NLC-Dac was diluted at 10 % with PBS (pH = 7.4) and incubated at 37 °C in a capped centrifugation tube.
Instead, we analyzed whether deviations accumulate in a certain centrifugation condition.
The traditional PRP preparation consisted in a slow centrifugation; platelets remain suspended in the plasma, while the leukocytes and erythrocytes are displaced to the bottom of the tube.
Biomass collected during 3 4 days was concentrated 100 times in a single centrifugation step and used for in vivo NMR experiments within 24 h after concentration.
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