The human telomere base sequence (Tel-22) prepared in Na+ buffer forms a G-quadruplex structure with the characteristic CD spectrum shown in Figure 1A.
The gradients were formed in a buffer containing 100 mM KCl, 5 mM HEPES-KOH pH 7.6, 0.2 mM EDTA and Proteinase Inhibitor Cocktail (Roche).
Additionally, the mmCD40Ig protein can bind hCD40L protein in phosphate buffered saline and form a stable combination in a size-exclusion chromatography assay using a Superdex 200 column.
The ability of these modified oligodeoxyribonucleotides to form triple helices in a buffer containing monovalent cations was studied by UV melting curves analysis, gel shift assay and restriction enzyme protection assay.
47 Lipid-rich myelin emulsified in aqueous buffers forms micellar structures of variable sizes, which can be separated by flow cytometry.
Consistent with this, Evans Blue aggregated at low micromolar concentrations in biochemical buffers, forming colloids with radii of 126 nm by DLS (Table 2).
Na-luminol and lucigenin (Santa Cruz Biotechnology) were dissolved in phosphate buffered saline to form 20-mg/ml and 2.5-mg/ml stock solutions, respectively.
Moreover, as the ionic strength is similar in the HEPES buffer and the 250 mM NH4Ac buffer, the fact that the protein is mostly in a dimeric form in HEPES buffer and mostly in a monomeric form in NH4Ac buffer, suggests a change of its protonation state between pH 7.5 (HEPES buffer) and 8.8 (NH4Ac buffer).
Prior to animal infections, bacteria were cultured overnight in LB broth, washed in a buffer containing 0.1 M HEPES (pH 8.0) and 0.9% sodium chloride, and resuspended in the same buffer to ~107 – 109 colony forming units (cfu) per ml.
Once in the matrix, calcium is buffered by forming reversible calcium phosphate complexes.
