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Extending the two common and two miRNA-specific primers produces a full-length 219 bp linear miRNA donor DNA (Additional Files 1 and 2), which is then used in a BP recombination reaction with the GRIM destination vector.
This assumes that the estimated change in BP is achieved, that the effect of non-adherence is negligible, that the change to (or inclusion in) a BP group completely and linearly defines the risk of morbidity and mortality, and that the amount of benefit does not diminish within any age group.
My journey to finding answers began in a BP gas station on 128th street.
On 20 April, a blow-out in a BP deepwater well kills 11 and spews millions of gallons of oil into the Gulf of Mexico.
In 2006, a leak in a BP pipeline spilled hundreds of thousands of gallons of oil in Prudhoe Bay, Alaska.
The gateway attB-flanked PCR product was recombined with a donor vector containing the corresponding gateway attP signals in a BP reaction to generate an entry clone.
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But in Planet BP -- a BP online, in-house magazine -- a "BP reporter" dispatched to Louisiana managed to paint an even rosier picture of the disaster.
Addressing the oil spill, Mr. Barbour has delivered briefings alongside Adm. Thad Allen of the Coast Guard, requested federal relief for businesses along the coast and appeared with his wife in a BP-financed advertising campaign encouraging tourists to keep coming to the Mississippi coast.
In a BP-Rex MD simulation replicas are controlled by various levels of a biasing potential to reduce the energy barriers associated with peptide backbone dihedral transitions.
SLV338 prevented cardiac remodelling to the same extent as losartan, but in a BP-independent manner.
This inhibition of inward Ca2+ current through L-type channels in vascular smooth muscle might result in a BP-lowering effect through a vasodilatory effect [ 42- 44].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com