We have established a binary expression system in which two genes, in opposite orientation and flanked by loxP sites, can be toggled successively between an inactive and active transcriptional state.
Each solution is a binary expression tree representation of a NN, similar to that shown in Figure 3.
The BIP-9 GUS BIP-9 GUSrs the promoter (2000 bp of the 5'-flineing region) of tharborsmic BIP-9 clone (soyBIP cDNA) fused thethe repromoterene GUS contained in a plant binary expression vector, pCAMBIA1381z.
A synthetic positive feedback system resulting in binary expression response was transduced via lentiviral transduction.
Furthermore, regardless of the choice of D*, this measure is still backwards compatible with the evaluation of the conditional entropy in binary expression data [ 15, 17, 18], in which case H is independent of D*, as illustrated in Figures 1D, 1E, 1F.
In summary, the binary expression of the two transgenic reporters in MacBlue× Cx3cr1 gfp/+ mice permits the identification of monocytes in tissues, and their distinction from other mononuclear phagocyte subsets as well as from NK cells.
In brief, after incubation of the binary expression vector with Cre recombinase in vitro, the reaction mixture was transformed into E. coli, and DNA prepared from single colonies to identify those clones that had undergone inversion between the two loxP sequences.
This relay system transforms a pattern of binary expression (Hh on in the posterior compartment, off in the anterior compartment) to the more complex nested patterns of expression of Dpp target genes.
After sequence confirmation, the LeNCED1 gene was introduced into a modified pGSA1403 binary expression vector 27 in which the CaMV-35S promoter was replaced with the rd29A promoter (Supplementary Figure 1).
To determine the role of Slfn4 in vivo, we over-expressed the gene specifically in macrophages in mice using a csf1r promoter-driven binary expression system.
Each location in the machine's memory had an "address," which was also coded by a ten-digit binary expression.
