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Single step purification resulted in 2 fold purification with 74% of recovery by ultrafiltration with 10 kDa cut-off.
Aliquots of crude cell extracts were diluted in 2 fold concentrated electrophoresis sample buffer containing SDS, heated and analyzed by SDS-polyacrylamide gelectrophoresissis (PAGE) [9], [10].
Aliquots of crude cell extracts were diluted in 2 fold concentrated electrophoresis sample buffer containing SDS, and processed for immunoblotting analysis [ 9, 10].
MRMs induced the glycolysis by enhancing the levels of two important regulators of glucose metabolism GLUT-1 and HK-II and resulted in 2 fold increase in glucose consumption and lactate production.
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GluR6 increased in Tg20 (6.5 fold) and in Prnp −/− (2.5 fold).
Finally, GABAA-γ2 was strongly up-regulated in Tg20 (4.5 fold increase) and increased in Prnp −/− (2 fold increase).
In contrast, GluR2 was down-regulated in Tg20 (0.5 fold) and Prnp −/− (0.7 fold) compared to wild-type.
The increase of LPAAT activity was more pronounced in slc1 (8 fold), than in ale1 (2.6 fold).
Hypercreatininemia was observed in L16 (2.7 fold vs W16, p<0.05).
All experiments were done in 5 fold repetitions.
Moreover, the combination of DCY84T and SiO2 treatments resulted in 2-fold greater fresh and dry weights of 60 DAS rice seedlings compared to Mock seedlings.
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