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The new IMS method is named AFADESI-IMS.
To assess the usefulness of the IMS method, FDC experiments using human skin were conducted, covering a concentration range of 0.32 69.57 μg/ml.
These improvements make it a simple, sensitive and robust PGS-UHPLC MS/MS method, and this method will become an attractive alternative to immunomagnetic sePGS-UHPLC MS/MSmethod and a useful diagnosthistool for retrospective detection of OPNA exposure with high confidence.
Interestingly, MOC31-bead-bound cells were detected in 25% of the donor samples, demonstrating that all the cells detected using the IMS method are not tumour cells.
To elucidate the sensitivity and specificity of the IMS method we performed, in sequence, IMS screening and RT-PCR analysis of clinical SLN samples.
This might have been because the calf excreted bacterial numbers below the detection limit of the IMS method, which was calculated to approx. 1 cfu/g faeces under these experimental conditions (unpublished data).
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We shall elaborate the derivation of the IM method in the forthcoming section.
Similarly, for 64 additive QTLs identified by IM method, only 111 of 1046 genes were annotated.
These methods include the Brovey transform (BT) [32], the P + XS [59, 60] and the intensity modulation (IM) method [61].
The IM method detected five additive QTLs (qK1.8, qK1.11, qK1.38, qK5.4, and qK6.4) for shoot K+ concentration.
In this paper, an improved Milstein (IM) method is developed, which successfully avoids solving nonlinear algebra equations encountered with implicit methods as mentioned above.
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