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We maintained reverse maps (from the imputed position in one of the Sanger strains back to the reference position) for determining gene, transcript, exon and SNP IDs.
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While large regions with a single genotype were consistent with some minor variation in imputed breakpoint position, the genotype of smaller regions varied between some replicates of samples covered in both the HincII and RsaI datasets (Additional file 8: Figure S7).
The numbers of reads carrying each possible allele (with respect to the positive strand) at all of the imputed heterozygous positions (after filtering using the Bisulfite sequencing data as described above) were determined for each histone modification.
The European American GWAS genotype data comprised 60 SNPs across this region, where we again imputed between positions 111 464 762 and 111 653 000 bp on chromosome 11.
Two of the patients with missing results for rs1337080 also had missing results for rs6152 but could be imputed for both positions, thus resulting in two common diplotypes.
In a second phase of imputation, 508 cases and 2910 controls were subjected to an imputation analysis to impute SNPs between positions 111 464 762 and 111 653 000 bp (dbSNP 126) on chromosome 11.
Missing genotypes of single nucleotide polymorphisms (SNPs) with known chromosomal positions were imputed by BEAGLE [ 9], and those with unknown chromosomal positions were discarded.
The 508 UK cases and 2910 UK controls were subjected to an imputation analysis to impute SNPs between positions 111 464 762 and 111 653 000 bp (dbSNP 126).
SNP with both known and unknown position were included and imputed, but the map order was randomized and SNP identity was recoded.
Completed education levels served to impute missing income positions (9.8% of cases) using the logistic regression method within PROC MI [ 22].
The genotypes at more than 17 million positions can now be imputed in any animal of the Fleckvieh breed with array-derived medium or high density genotypes.
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