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For each strain we selected 8 time points spread 6 h apart, from which we generated a curve of 376 imputed points.
To correct for variations in developmental stage speed between parasite lines, dynamic time warping [ 71] was applied to determine the optimal post-invasion time point (corresponding to the first sampling time point) along the IDC using the PCC between each pair of imputed points.
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Actual time points from the original time courses match imputed time points that are fairly evenly spread along the imputation-derived curve.
We observed that actual time points from the original time courses matched imputed time points that were fairly evenly spread along the imputation-derived curve, with correlations of average 83 91 % across the range of time points for each strain.
However, there is a slight curvature around the LOD, especially in period 3, that may reflect a lack of data between the LOD (0.023) and the minimum measured value > LOD in period 3 (0.07). Figure 2 also shows that the imputed data points overlapped substantially.
We found that 98% of imputed data points fell within the 95% confidence interval of observed data and there were no statistically significant differences of the slopes in the corresponding regressions [ 20, 21].
Because valid data were obtained in previous and subsequent months (indicating the sites were actively receiving traffic), we imputed these data points depending on whether they were after the credit card ban (n = 230) or before (n = 21).
Doing so, however, underestimates the variability of the data (because a single value is imputed for multiple points) and subsequently produces liberal estimates of statistical significance.
26 32 37 38 We imputed missing data points for pain scores, Oswestry disability index, and the composite outcome using the "last observation carried forward" method, which can underestimate effect sizes when "dropouts" occur because of lack of efficacy.
Among the combined data set with imputed missing data points, a total of 1818 genes with connectivity information of 104 TFs from genome wide location analysis were used to generate random sub-networks.
I apply an engineering thermal load model to impute balance point temperatures for residential households in the Puget Sound Energy Washington service territory.
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