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Using the 105 samples genotyped on both platforms, we assessed imputation accuracy; imputed genotypes for an SNP were retained only when none of the called genotypes were discrepant with the 1M genotypes.
Hickey et al. [ 41] imputed genotypes for non-genotyped individuals so that all individuals have genotypes for fitting a MEM.
Figure 4 shows a bubble plot of actual and incorrectly imputed genotypes for each of the six imputation methods.
Adjusting the proportion of correctly imputed genotypes for the expected proportion achievable by random imputation resulted in a much stronger relationship with the correlation between true and imputed genotypes, which was almost independent of the MAF, although SNPs with a lower MAF tended to show more variation in both measures of accuracy.
For example, Marchini et al., imputed genotypes for a UK sample using CEU HapMap haplotypes, and the imputation AR was high [5].
Erbe et al. [ 20] studied imputation to the HD-chip and found more than 20% incorrectly imputed genotypes for each SNP in a subset of 1231 SNPs.
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An imputation method that imputes genotypes for all animals in the pedigree for all SNP provides an alternative framework for single-stage genomic evaluations that avoids these problems.
Therefore imputation algorithms should be able to impute genotypes for all chromosomes.
Therefore, imputation algorithms should be able to impute genotypes for all chromosomes or regions.
Therefore, IMPUTE2 does not impute genotypes for ungenotyped animals in the pedigree.
The primary genotype data were used to impute genotypes for ∼17 M SNPs/indels using the 1000 Genomes Project (March 2012 release) as a reference panel and IMPUTE V2 (41).
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