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A research is also underway on concurrency improving transformations to produce a better gray-box model as a starting point for the scheduler stage.
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These precipitates can accommodate transformation strain, relieve constraint in adjacent austenite grains, and arrest cracks by extensive plastic deformation, thereby improving transformation ductility and shape memory effects.
Blanc et al. [ 22] showed that simultaneously increasing the auxin and cytokinin supply in the pre-culture medium prior to transformation of rubber tree using embryogenic cultures stimulated the development of active and fast growing cells, hence improving transformation efficiency.
It is noteworthy that in our study most progress in improving transformation efficiency was achieved by optimizing the production conditions of the embryogenic cultures used as target tissues for transformation rather than by optimizing the physical co-cultivation conditions.
However, improved transformation efficiencies of approximately 105 transformants/μg DNA with the same system of host/vector were achieved by Dornan and Collins [ 10] by altering the conditions under which the bacteria were grown prior to electroporation, e.g. by incorporating threonine in the medium, while cells of the early-log phase were used.
To determine whether Dam methylated DNA improves transformation efficiency, unmethylated plasmid DNA was Dam methylated in vitro and transformed into C. thermocellum.
In order to improve transformation efficiency and to achieve higher GFP expression levels as well as stable transformants, a new series of cloning vectors for ATM transformation was constructed.
In this work, for the first time, we prepared chitosan/pDNA nanoparticles by electrospraying methods to improve transformation process.
The kfoA and kfoC genes were sequentially cloned into plasmid pNZ8148 using L. lactis as intermediate host to improve transformation efficiency (Additional file 1).
Lloret et al. (2010) showed an improved transformation of DCF in the presence of different mediators such as syringaldehyde or 1-hydroxybenzotriazole.
These experiments showed equivalent or improved registration accuracy compared to other state-of-the-art (dis)similarity metrics and improved transformation consistency compared to pairwise mutual information.
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