Your English writing platform
Free sign upExact(3)
We have proposed a simple but promising strategy for improving the transformation efficiency of L. lactis and other hard-transformed microorganisms.
Recent studies that identified several plant genes involved in Agrobacterium-mediated transformation, and their over-expression in currently transformable species, suggest that this approach holds great promise for improving the transformation of recalcitrant, but agronomically important, crops.
Targetron plasmids were transformed into E. coli BL21 DE3), a dam+ dcm- strain, to remove Dcm methylation, thereby improving the transformation efficiency of C. thermocellum[ 55].
Similar(57)
Increasing the ratio of A. tumefaciens to target conidia to 1 × 104 improved the transformation frequency to 30 transformants per 1 × 104 conidia (Table 3, 48 h) on average; the single-copy integration of the T-DNA decreased to 30%, but the frequency of T-DNA integration into a single locus with the vector backbone typically increased to 55%.
The results indicated that the following conditions could improve the transformation efficiency.
Overexpression of some DNA binding proteins could improve the transformation efficiency.
But while the ability to gerrymander successfully has improved, the transformation in the structure of our politics has changed even more dramatically.
The new method improved the transformation efficiency of B. subtilis with linear integrative DNA nearly 700-fold compared with existing Bacillus transformation techniques.
In the present experimentation, a comprehensive and systematic study was performed to improve the transformation efficiency of kumquat, which was usually less than 4.1% in previous reports.
The results suggested that acid or erythromycin stress could improve the transformation efficiency of L. lactis through regulating gene expression of DNA binding proteins.
It can be concluded from this study that acidity is beneficial for conversion of asphaltenes but the hydrogenation active phase does not seem to be the main way to improve the transformation.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com