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Higher surface and bulk protein adsorption was observed for the matrices containing phosphonate groups, consequently improving the proliferation and attachment of MC3T3-E1 cells.
Exogenous therapeutic genes could be targeted to the rDNA locus of MSCs using the hrDNA vector described herein, and desirable number of the targeted cell could be obtained by improving the proliferation capacity of the MSCs using growth factors.
Finally, our results that ELF-PEMFs induction is quite efficient for improving the proliferation and differentiation of hABMSCs suggest that it may be a good strategy for hABMSCs-based tissue engineering applications with suitable scaffolds, especially fabricated by conducting biomaterials [ 58– 61].
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While research also revealed that epithelium devoted to clear microbes away from mucosal surface in mounting responses and improve the proliferation of intestinal probiotics.
Importantly, when applying these polymers for myotube formation, the HPLAAT significantly improved the proliferation of C2C12 myoblasts in vitro compared to HPLA.
The film layer was first used to release growth factors and it was shown that the release significantly improved the proliferation over 5 days.
Cell culture assays in low serum culture medium (LSCM) demonstrated that incorporating very small amounts of aFGF and bFGF into the (Hep/Chi n multilayers significantly improved the proliferation of the NIH-3T3 fibroblasts.
The results indicated that moderate shear stresses (0.02 0.19 Pa) generated in the bioreactor improved the proliferation of rBMS cells to around 1.3 times of the static control with well maintained calcium deposition ability of the cells.
The MSCs isolated from unconventional model organisms are being studied for their potential to heal different tissue defects and injuries and for the development of scaffold compositions that improve the proliferation and differentiation of MSCs for tissue engineering.
These scaffolds also greatly improved the proliferation of rat pheochromocytoma 12 (Pcellscells, and significantly promoted their differentiation and neurite growth along the nanofibrous alignment in the presence of nerve growth factor (NGF).
Furthermore, it was shown by western blot assay that the culture of chondrocytes in microcavitary hydrogel could improve the proliferation of cells potentially by inducing the Erk1/2-MAPK pathway.
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