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Notably, fusing target proteins to a highly expressed bacterial protein has proven particularly effective for improving the expression level of membrane proteins in E. coli [21].
Moreover, the two regulatory elements were combined and applied in the BEVS, thereby improving the expression level of PCV2 VLPs.
With the baculovirus preparation process becoming more convenient, researchers will focus on improving the expression levels of exogenous proteins by changing the baculovirus genome.
The substitution of part of the N terminus has been critical both in improving the expression of this enzyme as a recombinant protein and in achieving its crystallisation.
Using a reporter protein downstream of the synthetic gene, allows the selection of clones with random mutations improving the expression or the folding of the protein of interest.
This study suggests a simple strategy for improving the expression and immunogenicity of HIV subtype-specific envelope antigens as plasmid or vector-borne immunogens.
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Many efforts have been made to improve the expression of heterologous genes in the BEVS.
This result indicated that using the rich media improved the expression level of SrtA.
Therefore, more endeavors should be made to improve the expression level of this important enzyme.
In this study, we described a strategy to improve the expression efficiency using synthetic double promoter.
The results show that the two regulatory elements could also effectively improve the expression of PCV2 VLPs in Sf9 cells.
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