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By further improving the assay condition and format, we are able to detect fewer than ten targets in 1 h and to apply successfully this method for detection of Epstein Barr virus in human lymphoma specimens.
Currently, we are focused on further improving the assay sensitivity and SNP discrimination ratio and extending its application to clinical samples.
Because the company is improving the assay with time, different kits were used for the characterization of all dNFs: Salsa MLPA Kit P122 versions P122, P122-B1, and P122-C1.
These advantages, along with the possibility of improving the assay format, suggest that it may be worthwhile to use this assay to reevaluate the frequency with which known tumor-associated proteins are detectably antigenic in cancer patients.
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These also call for improving the assays' amenability towards automation and miniaturization because most current assays still require the use of expensive and complex instrumentation and complex procedures, limiting their potential in rapid diagnosis.
Using these clones eliminated background and greatly improved the assay sensitivity and reproducibility, crucial factors for the high throughput analyses.
However, further data accumulation is still needed to improve the assay performance because the eROS assay provided false predictions for some compounds.
Studies to improve the assay performance in the field were performed, showing that assay activity could be preserved for up to 2 months.
It was also established that the use of methanol as cosolvent significantly improved the assay sensitivity through the stabilization of the ternary complex.
Efforts to improve the assay sensitivity of analgesic trials could reduce the rate of falsely negative trials of efficacious medications and improve the efficiency of analgesic drug development.
Finally, using P3 and P5 with longer binding sites results in increased overall sensitivity of the reaction, showing that increasing ligation efficiency can improve the assay overall.
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