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For most of these targets, the particular short chain MAG in question was essential to the success of the project by enabling crystallization in the first instance and/or improving crystal quality to the point of providing a useful structure.
It was also found that annealing treatment was in favor of forming the mixed crystal and improving crystal quality.
XRD and TEM results disclosed that the composition x can be precisely controlled by adjusting growth time ratio of ZnSe to CdSe; meanwhile, annealing treatment under a suitable temperature is beneficial for forming ternary alloys and improving crystal quality.
This study is conducted with the aims of improving crystal packing and structure resolution.
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Cation exchange complements previously reported postcrystallization dehydration of protein crystals and represents a potentially general strategy for improving crystals of large RNAs.
To improve crystal size and morphology, crystallization droplets were set up as described above with xylitol (3%, w/v), pre-equilibrated for 24 h, and then seeded with serially diluted suspensions of crushed microcrystals.
In order to improve crystal quality, a large number of crystallization conditions must be explored to increase the number and quality of protein crystals produced.
As in the case of laccase, nanospheres could be used as additives in crystallization to control nucleation and to improve crystal quality as an alternative for seeding.
One approach to improve crystal quality is the use of DARPins as crystallization chaperones.
However, the crystallization probability was low, and even when we obtained a crystal, it diffracted X-rays up to only 2.0 Å. (14) When we applied the same crystallization conditions to the counterdiffusion method on the ground, it yielded similar results of low crystallization probability, although the counterdiffusion method has been reported to improve crystal quality in some cases.
All four approaches were found to improve crystal size and/or shape compared to simple unseeded or seeded linear cooling crystallizations.
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