Effective siRNA loading and improved transfection efficiency into cells represents a key problem.
In addition, modifying the electroporation parameters improved transfection efficiency and expression of plasmid-derived IL-12p70, as well as its downstream effector IFN-γ in vivo.
These complexes were designed to increase the stability of mRNA, to improve transfection efficiency and to reduce cytotoxicity.
A variety of DNA formulations, mainly based on cationic liposomes or polymers, has been investigated to improve transfection efficiency in in vitro assays.
Together, these unique features of bPOEAA2 enabled markedly improved transfection efficiency in cells in physiological medium and much lower cytotoxicity than bPEI.
We hypothesized that this delivery strategy would facilitate improved transfection efficiency while eliminating the toxicity seen for many vectors due to membrane destabilization.
Enantiospecific delivery of siRNA to Huh 7 cells is seen by one 'enantiomer' of the polymers delivering siRNA with significantly improved transfection efficiency and reduced toxicity compared to the 'enantiomeric' polymer and commercially available transfection reagents.
These results indicate that hydrophobic modification of low-toxicity PEI without reduction in primary amine content is an effective strategy for improving transfection efficiency of polycation-based non-viral vectors while maintaining low toxicity.
Zhang PC, Wang J, Leong KW, Mao H (2005). Ternary complexes comprising polyphosphoramidate gene carriers with different types of charge groups improve transfection efficiency.
After confirming EF-1α promoter activity in ES cells (Figure 3C), we used the Amaxa electroporation transfection system to improve transfection efficiency in ES cells and MEFs.
