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The combination of multiple protease digestion with ion mobility separation significantly improves sequence coverage and absolute quantification of this 14.8 kDa r-protein.
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In summary, post-amplification normalization improves sequencing efficiency and genome assembly, and in its absence, bioinformatic normalization may be required for de novo assembly.
Now we demonstrate that a knowledge of these differences improves sequence-to-structure alignment.
In addition, high coverage (>30x 40x) improves sequencing accuracy.
Incorporation of multiple mapped reads into the calculation improves sequencing depth (up to 25%) (Chung et al. 2011) and helps identify binding sites in regions containing repetitive sequences.
Moreover, the paired-end sequencing strategy of RNA-seq further improves sequencing efficiency and extends short read lengths for better understanding transcriptome [ 16].
For example, Mann et al. [18] presented a semi-supervised CRF to improve sequence segmentation and labelling.
Standard and long range PCR was used to close gaps, improve sequence quality and resolve any remaining base-conflicts.
To improve sequence coverage, we used a size selection procedure that removed cDNAs shorter than 1.3 kb in length.
Additional directed sequencing was performed as necessary to improve sequence quality genome-wide to a minimum base call error rate of 1 in 10,000 (i.e. Q40).
In summary, improved sequence maps and technical capabilities are necessary to increase success in identifying and validating common genetic variants associated with telomere length homeostasis.
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