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Our results demonstrate that probe selection improves gene expression estimates from exon arrays.
Based on the existing information about the structure of operons, we propose a hierarchical Bayesian model which improves gene expression estimation by borrowing information from genes within the same operon.
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However, a significant body of literature suggests that synonymous codon usage beyond the initiation region can impact expression [3], [8], [9]; for example E. coli strains over-expressing rare tRNAs can significantly improve gene expression [12] [14].
This study highlights the potential in using a combination of delivery strategies to improve gene expression.
In vivo SSTR2-based reporter imaging can be used to compare methodologies for improving gene expression.
In addition, the introduction of RGD group further improved gene expression level.
Increasing the molecular weight of statistical polymer to some extent improved gene expression efficiency.
The galactose-based hyperbranched glycopolymer of ∼30 kDa or lower show improved gene expression at varying polymer/plasmid ratios.
In summary, our study demonstrates improved gene expression when using angiotensin II in combination with adenovirus upon intra-arterial delivery in a large animal model.
The formulated nanoparticles enhance cellular uptake of miRNA, pronounce upregulation of miR-26a, induce cell cycle arrest, and improve gene expression activity compared with PEI and commercial liposome.
Using a SSTR2-based reporter, we assessed whether angiotensin II can improve gene expression by adenovirus upon intra-arterial delivery in a rabbit VX2 tumor model.
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