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Isolation and expansion of adult HACs under physiological tonicity (380 mOsm) improves expression of chondrogenic markers on mRNA and protein levels.
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In this study we attempted to improve expression of two difficult-to-express coding sequences (araH and narK) for membrane transporters.
Nevertheless, this study showed that supplementation of the CNGB3 transgene improved expression of the CNGA3 subunit and that the expressed CNGA3 was able to traffic correctly into the outer segments.
Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis.
Gene optimization has been used to improve expression of the antibody master genes in the library context.
This study represents a systematic approach to broadly identify genetic loci that can be used to improve membrane protein expression, and our method can be used to improve expression of any protein that poses a cellular burden.
The methods discussed include modified gene amplification methods, modifying vectors to improve expression of the therapeutic gene and improving the method of selecting for high-producing cells.
As a result, the Δmms13 strain synthesized BacMPs with significantly improved expression of two human proteins, thyroid-stimulating hormone receptor (TSHR) and the class II major histocompatibility complex (MHC II) molecules.
In particular, our results support the notion that full codon optimization may be unnecessary to improve expression of viral genes rich in E. coli rare codons; using a strategically modified host cell could provide a simpler and cheaper alternative.
In this process, improved expression of pou5f1 may act.
For these reasons, improving expression of emotion for music robots is the main focus of this study.
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