Sentence examples for improves expression levels from inspiring English sources
Codon-optimization using the UpGene algorithm improves expression levels of the antigen in infected mammalian cells and consequently enhances immune responses against the antigen in vivo[16].
Previous work had shown that, in Chlamydomonas, adaptation of the codon usage of trangenes significantly improves expression levels (Fuhrmann et al. 1999, 2004).
The generation of a scAb, which had a fused human kappa light chain constant domain (Ck), was shown to significantly improve expression levels in Escherichia coli.
The E. coli thioredoxin at the receptor C-terminus was found to improve expression levels [6] and a C-terminal deca-histidine tag allowed the efficient capture of the NTS1 fusion protein by immobilized metal affinity chromatography (IMAC) [7].
The reporter contains artificial introns and the C. elegans unc-54 3'UTR to improve expression levels in C. elegans.
The percentage of glycosylated recombinant AcrA protein, produced in E. coli, was increased by improving expression levels of oligosaccharyltransferase, pglB.
Trials with a GST fusion expression system driven by the stronger T7 promoter did not improve expression levels (results not shown).
Diploidization is another promising strategy to improve expression levels of heterologous genes and enhance the fermentation ability of S. cerevisiae [ 12- 15].
It is therefore possible that hMSCs from CAD+DM patients modified with Bcl-2 gene may have improved expression levels of Bcl-2 protein and enhanced ability to protect the ischemic myocardium.
His-Sumo-N-GF showed a highly improved expression level (>20 mg/L in E. coli) and solubility when compared to other deletion constructs with the N-terminal 83-aa domain (Fig. S1C).
The expression levels of all the other deletion constructs (1 387, 1 413) were greatly reduced, but ∆1 83 exhibited a highly improved expression level (Fig. S1B), indicating that the N-terminal 83-aa domain of Mx2 may cause low expression and instability in solution (Table S1).
