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One such approach is to improve the DNA plasmid by introducing codon optimization in the DNA plasmid that improves expression and immunogenicity in animals.
Codon-optimization using the UpGene algorithm improves expression levels of the antigen in infected mammalian cells and consequently enhances immune responses against the antigen in vivo[16].
Isolation and expansion of adult HACs under physiological tonicity (380 mOsm) improves expression of chondrogenic markers on mRNA and protein levels.
Previous work had shown that, in Chlamydomonas, adaptation of the codon usage of trangenes significantly improves expression levels (Fuhrmann et al. 1999, 2004).
To study whether the cross-hybridization correction improves expression estimates, we compare GeneBASE and GeneBASE-xhyb to estimates obtained by Solexa sequencing of RNA sequences for mouse liver, skeletal muscle and brain samples.
Contrasting detrimental signalling changes in more severe disease states, we show that dietary obesity with mild hyperleptinaemic significantly improves expression and activation of Akt, phospho-activation of eNOS and Erk1/2, and phospho-inhibition of GSK3β (Figs 4- 6) in myocardium from insulin-insensitive animals.
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In this study we attempted to improve expression of two difficult-to-express coding sequences (araH and narK) for membrane transporters.
In this process, improved expression of pou5f1 may act.
In addition, all clones displayed improved expression characteristics in Escherichia coli.
Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis.
For these reasons, improving expression of emotion for music robots is the main focus of this study.
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