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It is expected that such improvements of the protocol will continue to drive the utility of this assay in the product safety assessment.
However, there are still additional improvements of the protocol before satisfactory artificial fertilization is achieved, such as routines to further minimize the handling stress.
Because the available methodological studies of the OxHLIA appear to have reviewed and extended its procedure into a robust methodology, no improvements of the protocol have been performed.
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However, only a few studies aimed at an improvement of the protocol for mass propagation.
The logical improvement of the protocol in this case was to wash the immunoprecipitated DNA without spin-columns.
Several experts agree on the need for improvement of the protocol feasibility phase, and even categorize it as one with the greatest potential for improvement [ 8].
The putative underlying physiological processes are discussed and hypotheses on improvement of the protocol for in vitro somatic embryogenesis in Cyclamen persicum are deduced.
However, further improvement of the protocol is needed for cell-based therapies such as transplantation, because undifferentiated cells could cause tumor formation.
Finally, comparisons between the proposed protocol and the previous protocols are given to demonstrate the improvements of the proposed protocol.
This might be of high relevance in regard to the reduced regeneration rates after cryopreservation and could lead to improvement of the protocols.
We now describe improvements of the transduction protocol designed to preserve HSC properties and two modifications of the previously described TRIP-CMV vector.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com