The rapid improvements of sequencing technologies and sequence analysis tools in the past decade has extended our ability of generating and mining genomic information to several levels beyond what was possible more than a decade ago (Gomez-Escribano et al. 2016; Shendure and Lieberman Aiden 2012) when the first version of the N. punctiforme ATCC 29133 genome was released.
In addition, the numbers of reference sequences in databases will continue to increase with further improvements of sequencing technologies.
This result shows that SIH will be able to infer longer haplotypes in accordance with improvements of sequencing technologies.
Database URL: http://arabidopsis.usc.edu/ The drastic improvements of sequencing and genotyping technology, driven by the human genome project, have made it easier than ever to obtain genome-wide genetic data.
While several statistical algorithms have been developed to infer unknown haplotypes from genotype data [ 10- 12], the improvements of sequencing technologies will enable researchers to assemble haplotypes from sequencing data with very high accuracy (for examples of existing assembly algorithms, see Bansal et al. [ 13], Bansal et al. [ 14], and Schatz et al. [ 15]).
The 43 760 predicted protein-coding loci should be examined by further improvement of sequence assembly and gene prediction.
With the improvement of sequence coverage, sequence quality and mapping algorithms, in silico SNP detection will become increasingly reliable in the future.
The improvement of sequence databases, including EST databases, and repeat databases will be instrumental in the successful design of these probes for cross-species hybridizations.
Our study not only provides essential information for the improvement of sequence assembly but also offers molecular tools for cucumber genomics research, comparative genomics and evolutionary study.
The use of the web interface within the PubMLST Neisseria database enables a process of community annotation whereby different members of the community can participate in the maintenance and improvement of sequence annotation and interpretation.
