Sentence examples for improvements in the tetracycline system from inspiring English sources

Improvements in the tetracycline system have led to the development of tetR targeted repressors such as tetR KRAB [ 24] and tTS [ 25], which have been shown to reduce efficiently basal expression from the P tet regulated promoters in vitro and when codelivered to skeletal muscle [ 26, 27].

Efficient regulated expression was achieved in a wide variety of cell lines, including 293T cells in which the tetracycline system has previously been shown to function poorly [ 29].

Importantly, in those cell lines the tetracycline system was still fully functional, as shown by tetracycline induction of an unrelated transgene (data not shown).

Recently, inducible expression systems have been described for the use in ES cells, mainly based on the tetracycline system [ 25].

The tetracycline system has previously been utilized in CIA gene therapy for regulated expression of vIL-10 [ 27, 36].

In the 10% of mice in which relapse occurred, tumors had either escaped conditional regulation by the tetracycline system or had undergone a presumed secondary genetic event.

Several pharmacologically regulated systems of gene expression have been developed, including the tetracycline system, which uses the bacterial components of tetracycline resistance in a synthetic system that functions efficiently in eukaryotic cells [ 7, 8].

Flp-In T-REx 293 cells were transfected with pcDNA5/FRT/TO-PLM and pOG44 and then selected with hygromycin to generate cells expressing PLM in the tetracycline inducible system.

Regulated expression with the original tetracycline system is optimal in stably transfected cells, whereas expression in transiently transfected cells is approximately 50- to 100-fold [ 9- 11].

In the tetracycline (Tet) inducible system, the prokaryotic protein, Tet, binds to a specific DNA sequence called tetO in response to tetracycline or doxycycline (dox) [ 4].

But improvement in the public-school system is not inevitable.