Refined approaches such as a deconvolution method [26] or analytical modeling [27, 28] could bring an improvement in the activity quantification compared to uniform distribution subtraction.
Improvements in quantification of signal and quantification of bacteria in small tissue samples may enable measurement of bacterial numbers in individual images.
While this may suggest that ultrasound demonstrates better test-retest reliability than MRI, it should also be noted that significant improvements have been made in the quantification of cartilage measurements by MRI [ 35] since that study took place.
Improvements in the methods of multiplexed quantification of gene expression thus hold great promise also for this emerging field of studies, particularly when such methods are suitable for small-sample or single-cell analysis as the one described in this work.
As the improvements in quantification accuracy achieved by explicitely modeling biases have shown to be moderate [Using rQuant with an l2-loss function, an explicit model of the transcript length bias increased the pearson correlation coefficient by 0.4 1.7 percentage points (Bohnert, 2011, Table 3.1)], we decided not to incorporate this into our model.
This need will drive research leading to improvements in image quantification and to more sophisticated image analysis.
Improvements in PET/CT quantification include partial volume corrections methods to overcome limitations in PET spatial resolution, most commonly through sharpening of small image features with a point spread or imaging system response function[32].
Paired-end sequencing produces modest improvements in isoform quantification (Table S12); however, in our experience current enzymatic fragmentation protocols yield fragments that are too short to provide a clear benefit from paired-end sequences.
As the NSAF depends on peptide coverage, one could envision improvements in r-protein quantification throughout the entire molecular weight range by that and other related approaches, which would not necessarily require additional ion mobility separation of the multiple protease digestion mixture.
Recent advances in real-time PCR analysis have led to significant improvements in the accuracy and simplicity of DNA quantification and gene expression analysis.
