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The rapid improvement of sequencing technologies has enabled a number of different sequencing-based applications like genome resequencing, RNA-Seq, ChIP-Seq and many others [ 2].
Besides, additional minor miRNAs (also called miRNA star) can be also detected with the improvement of sequencing depth.
This approach, together with continuing improvement of sequencing technology, allows medical genomics to be a routine diagnostic method for rare Mendelian disorders in the near future.
The fields of genomics and transcriptomics maintain their fast development thanks to the improvement of sequencing technologies and associated bioinformatic tools for assembly and annotation.
The subsequent improvement of sequencing technologies made feasible the metagenome shot-gun sequencing of environmental samples; however, most environmental communities are far too complex to be fully sequenced in this manner.
However, the number of causative genes is growing very rapidly thanks to the improvement of sequencing techniques, and the classical testing of one gene after the other is progressively being replaced by diagnostic kits allowing multiple genes to be tested in parallel.
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The 43 760 predicted protein-coding loci should be examined by further improvement of sequence assembly and gene prediction.
With the improvement of sequence coverage, sequence quality and mapping algorithms, in silico SNP detection will become increasingly reliable in the future.
The improvement of sequence databases, including EST databases, and repeat databases will be instrumental in the successful design of these probes for cross-species hybridizations.
Our study not only provides essential information for the improvement of sequence assembly but also offers molecular tools for cucumber genomics research, comparative genomics and evolutionary study.
The use of the web interface within the PubMLST Neisseria database enables a process of community annotation whereby different members of the community can participate in the maintenance and improvement of sequence annotation and interpretation.
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