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To overcome the lability of LipC, we developed a rational design system to modify specific sites on the enzyme structure to obtain an improved variant of the lipase bearing higher thermal stability, but without loss of its cold-adapted properties.
An improved variant of the CAI peptide was generated by "hydrocarbon stapling", and the resulting peptide, NYAD-1, disrupted formation of both immature and mature virus-like particles in cell-free and cell-based in vitro assembly assays.
The resulting multiobjective problem is solved by a genetic algorithm following an improved variant of the so-called NSGA II algorithm.
In the following month an improved variant of the Ariane V rocket launched Europe's heaviest spacecraft, the Jules Verne Automated Transfer Vehicle (ATV), which carried 7,700 kg (17,000 pounds) of supplies to the ISS.
We introduce a new variant called Intelligent Bidirectional-RRT* (IB-RRT*) which is an improved variant of the optimal RRT* and bidirectional version of RRT* (B-RRT*) algorithms and is specially designed for complex cluttered environments.
The EDCA scheme is a contention-based mechanism which itself is an improved variant of the basic IEEE802.11 DCF.
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An improved variant of this approach involves combining the top hits instead of only using the best one [ 8].
All of these designs were improved variants of the K-GF series, which itself introduced many notable design innovations such as box-frame construction, intensively cooled cylinder components and hydraulically actuated exhaust valves.
After one round of site-directed saturation mutagenesis, improved variants of the two best designs, RA114 and RA117, exhibited among the highest kcat (> 10− 3 s− 1) and kcat/KM (11 25 M− 1 s− 1) values observed for retroaldolase designs prior to comprehensive directed evolution.
In the 21st century, improved variants of those military weapons remained in use around the world.
Random mutagenesis by error prone PCR (ep-PCR) was used to get thermostability improved variant of TtAG.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com