Sentence examples for improved transformation from inspiring English sources
However, improved transformation efficiencies of approximately 105 transformants/μg DNA with the same system of host/vector were achieved by Dornan and Collins [ 10] by altering the conditions under which the bacteria were grown prior to electroporation, e.g. by incorporating threonine in the medium, while cells of the early-log phase were used.
These experiments showed equivalent or improved registration accuracy compared to other state-of-the-art (dis)similarity metrics and improved transformation consistency compared to pairwise mutual information.
Although shape memory is improved, transformation reversibility remains limited by the necessary misfit dislocations that accommodate the atomic spacing differences in the interface.
Moreover, to perform integrations on irregular elements, an adaptive integration scheme considering both the element shape and the projection point associated with the improved transformation is proposed.
Finite-element simulation of quenching incorporating the improved transformation kinetics was performed to predict the temperature, microstructure, residual stress and distortion of a plain medium-carbon steel cylinder.
To explore the effects of overexpressing wild-type, mutant, and exogenous adhEs, we developed a new expression plasmid, pDGO144, that exhibited improved transformation efficiency and better gene expression than its predecessor, pDGO-66.
To determine whether Dam methylated DNA improves transformation efficiency, unmethylated plasmid DNA was Dam methylated in vitro and transformed into C. thermocellum.
In order to improve transformation efficiency and to achieve higher GFP expression levels as well as stable transformants, a new series of cloning vectors for ATM transformation was constructed.
These precipitates can accommodate transformation strain, relieve constraint in adjacent austenite grains, and arrest cracks by extensive plastic deformation, thereby improving transformation ductility and shape memory effects.
In this work, for the first time, we prepared chitosan/pDNA nanoparticles by electrospraying methods to improve transformation process.
The kfoA and kfoC genes were sequentially cloned into plasmid pNZ8148 using L. lactis as intermediate host to improve transformation efficiency (Additional file 1).
