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Using these clones eliminated background and greatly improved the assay sensitivity and reproducibility, crucial factors for the high throughput analyses.
Moreover, we greatly improved the assay sensitivity by the employment of AuNPs labelled with a high concentration of ECL signal probes carried by cysteamine.
It was also established that the use of methanol as cosolvent significantly improved the assay sensitivity through the stabilization of the ternary complex.
The test rapidly (20 min) detected 0.50% (w/w) bovine or ovine meat; Extraction Solvent was the most efficient extractant tested; EDTA coupled with heating (100 °C, 10 min) improved the assay sensitivity; and all the mixing methods achieved the same results.
We used a modified radioactivity-based detection protocol that greatly improved the assay's reliability and sensitivity [15].
This is in contrast to the pAb developed previously in this laboratory where the heterologous hapten assay format greatly improved the assay sensitivity.
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It has been reported that the presence of poly ethylene glycol) (PEG) as a linker between the surface and the peptide improved the assays [ 18– 20].
However, further data accumulation is still needed to improve the assay performance because the eROS assay provided false predictions for some compounds.
Studies to improve the assay performance in the field were performed, showing that assay activity could be preserved for up to 2 months.
Efforts to improve the assay sensitivity of analgesic trials could reduce the rate of falsely negative trials of efficacious medications and improve the efficiency of analgesic drug development.
Finally, using P3 and P5 with longer binding sites results in increased overall sensitivity of the reaction, showing that increasing ligation efficiency can improve the assay overall.
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