Sentence examples for improved stringency from inspiring English sources
Therefore, to reach improved stringency in CNV calling and to utilize methods implemented in both ExomeCopy and ExomeDepth, we designed an algorithm (ExCopyDepth) combining these two programs (Flow and implementation of ExCopyDepth; Additional file 1: Figure S2).
We also described a novel MBD protocol with improved stringency by exploiting the competitive binding of non-human carrier DNA to sequester excess MBD.
Since we are interested in detecting shorter CNVs with improved stringency, we combined different methods implemented in each of these programs in a modified algorithm, aiming to reach higher stringency compared to ExomeCopy and lower stringency compared to ExomeDepth.
Nevertheless, our thresholds were on the higher side to improve stringency.
To our knowledge, this is the first description of exogenous blocking DNA being used to improve stringency of MBD-based assays.
Furthermore, we improved the stringency of the prediction by considering certain quantitative scores.
As shown in Figure 4, it was hard to differentiate CD24 heterozygotes from homozygotes at low stringency, but separation gradually improved as the stringency was raised, as would be expected for a probe pair that is functioning correctly.
To further improve selection stringency, a series of weakened SV40E promoters have been generated and used to modulate plasmid-based GS expression with the intent of manipulating GS-CHO selection, finely adjusting the balance between GS expression and GS inhibitor (MSX) levels.
To improve the stringency of our analysis we considered genes as changed only if they had a t-test and a TNoM p-value <0.05 and a fold ratio >2 as previously described [20].
Another expression vector engineering approach is to improve selection stringency [ 31].
