Recently, improved sequences for the N-terminal and C-terminal capping helices have been described.
With the improved sequencing technology, the last two years have seen the development of NGS combining restriction-site associated DNA (RAD) for SNP testing [ 23].
To enable this work, we needed to construct bioinformatic tools, which we expect to be of broad utility to the research community, especially as with the advent of improved sequencing methods, the predicted structures of genes are constantly evolving.
With improved sequencing methods, the next phase of the A. thaliana 1001 Genomes Project should lead to the complete reconstruction of alleles, starting with reference guided assemblies18,50.
Improved sequence specificity of the DNA cytosine methyltransferase HhaI was achieved by disrupting interactions at a hydrophobic interface between the active site of the enzyme and a highly conserved flexible loop.
The longer average read length was mainly due to the improved sequencing chemistry.
In the coming years, larger collaborations, improved sequencing technologies and dissection of the phenotype hold considerable promise for further advances.
One can perform RD-PCR followed by DNA shuffling to improve sequence diversity of the library.
There is a great deal of interest in more accurate techniques for sequencing viruses, but as the results here make clear, there isn't really much benefit in improving sequencing accuracy if the accuracy is already > 1/N.
To improve sequence similarities, we applied the RACE-PCR technology and have sequenced the full cDNA sequences of ESTs (one EST for each cluster) with similarities to antibacterial peptides, lysozymes, PGRPs and Tollip.
