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Improved sequence specificity of the DNA cytosine methyltransferase HhaI was achieved by disrupting interactions at a hydrophobic interface between the active site of the enzyme and a highly conserved flexible loop.
In summary, improved sequence maps and technical capabilities are necessary to increase success in identifying and validating common genetic variants associated with telomere length homeostasis.
This assay offers a broad dynamic detection range and improved sequence variation tolerance.
We also tested whether improved sequence assemblies could be generated by separately altering certain Phrap parameters.
Short truncated compounds (minimal domain), chimerical constructions and improved sequence analogs have been reported.
Combining the Roche 454 long reads of ~ 250 bp and ~ 84 bp read length from the Illumina/Solexa method improved sequence alignments.
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With improved sequencing capacity, demand for increased sample preparation throughput has risen.
With improved sequencing methods, the next phase of the A. thaliana 1001 Genomes Project should lead to the complete reconstruction of alleles, starting with reference guided assemblies18,50.
In the coming years, larger collaborations, improved sequencing technologies and dissection of the phenotype hold considerable promise for further advances.
With the recent release of a new version of the diploid Fragaria vesca genome v2.0.a1 (denoted as Fvb 33,34,35 and improved sequencing technologies, synteny between Rosa and Fragaria can now be examined at a much higher resolution.
In addition, the development of faster, cheaper and improved sequencing methods has made genomics a tool that has a place in the workflow of a routine microbiology laboratory.
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