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Twinscan exploits cross-species homology between closely related genomes to produce improved gene models.
EuGene is another gene prediction program developed to make use of comparative genomics for improved gene models.
A fasta file with the improved gene models is available in the supplementary material, Supplementary Material online.
Along with the improved gene models we discover new, more accurate means of identifying signal peptides in proteomics data.
An additional level of complication is introduced by the instability of identifiers, which are changed, for example, as a result of improved gene models.
The discrepancy between the two studies is due to improved gene models provided by the more recent release of the P. trichocarpa Phytozome 7.0 database (http://www.phytozome.net).net
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Clearly, there are still changes to expect on the status of pseudogenes or genes for members of the C2H2-ZNF family in years to come based on the release of improved genome assemblies and detailed manual curation of individual genes for improved gene model and gene prediction.
"MAKER" (Cantarel et al. 2008) was used to construct an improved gene model for the genomic region which contained the TRP-N locus by using the alignments of the TRP-N proteins of Xenopus tropicalis, D anio rerio, D. melanogaster, and Caenorhabditis elegans.
To validate and improve gene models, we manually curated inaccurately annotated genes: 335 genes were manually added, and 86 genes were reclassified as pseudogenes.
In addition, our sequence data confirm or improve gene models, splice predictions and annotation of the soybean genome.
We analyzed the cognate transcriptome data in the CATMA resource and carried out data-mining to characterize novel genes and improve gene models.
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