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Besides, the smooth texture of the soybeans after germination increased the soy protein content by more than 40% in comparison to the raw soybeans, owing to the improved extraction efficiency of the soy proteins [23].
Optimized designs permitted as high as factor of six improved extraction efficiency.
The development and application of alternative green technology to replace conventional extraction methods with improved extraction efficiency and low environmental impact for the determination of natural bioactive compounds is therefore, highly important.
In this way, the improved extraction efficiency and/or de novo biosynthesis caused by the incomplete sprouting may have altered the total abundance of phenolic compounds in the pickled soybeans and pickling solutions.
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Numerous methods have been developed to improve extraction efficiency, use less solvent and short time.
Ultrasound has been used to improve extraction efficiency in terms of time needed and total polyphenol content.
Apart from the salting-out effect which improves extraction efficiency, it also leads to alter the physical properties of the Nernst diffusion film in the interface of aqueous and organic phases; consequently, the diffusion rate of analytes into the solvent was reduced [44, 45].
The sonication not only improves extraction efficiency and reproducibility, but also breaks large-molecule nucleic acids into small fragments that can be removed by subsequent precipitation steps.
Additionally, it was later found that sonication of cultures did not improve extraction efficiency, with extraction of unlysed (i.e., nonsonicated) culture offering the added benefit of decreased emulsion formation.
10) OPTIONAL: Add 1 μl of 1 μg/μl MS2 Bacteriophage carrier RNA solution to improve extraction efficiency [ 41]. 11) Incubate TRIzol LS/biofluid mixture for 5 minutes at room temperature.
The optimization improved the extraction efficiency of the more polar analytes reaching 93.9% for 4-epioxytetracycline and 95.8% for oxytetracycline at 100 μg L−1.
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