Sentence examples for improved copy number from inspiring English sources

The proposed normalization strategy, as well as the adaptive sample-specific level scaling, provides powerful and convenient means for improved copy number analysis using aCGH.

We recently demonstrated a high-density array for self-digitization of sample volumes that could be adapted for improved copy number resolution in this device.

Samples were run in triplicate and assays in sextuplicate, which generated high numbers of replicate data points allowing for improved copy number resolution to an extent not possible by conventional qPCR (Weaver et al., 2010; Whale et al., 2012).

Taken together, we demonstrate that copy number populations in aCGH data should be accounted for during normalization and that the proposed normalization strategy, as well as the adaptive sample-specific level scaling, provides powerful and convenient means for improved copy number analysis using aCGH.

We hybridized DNA from LAU-Me275 to Illumina 1M SNP arrays and analyzed the signals using the OverUnder algorithm [16], which uses minor allele frequencies in heterozygous loci to improve copy number estimation.

The package includes novel methodology on (i) filtering, reducing false positives, and (ii) GC content correction, improving copy number profile quality, especially under great read coverage and high correlation between GC content and copy number.

Several methods have been described that estimate, and then correct for, tumor cellularity in SNP array data in order to improve copy number profiles [ 2– 5] or in DNA sequencing data for mutation calling [ 6].

Additionally, we have not fully utilized the multiplexing capacity of MLPA [ 11] or Triplex PRT [ 5] which would further improve the copy number typing of these systems.

A better understanding of the replication mechanism of pRQ7 will help to formulate new strategies on improving the copy numbers of pDH10 and pDH12.

While advances in microarray technology have improved detection of copy number alterations, the increase in the number of measured signals, noise from array probes, variations in signal-to-noise ratio across batches and disparity across laboratories leads to significant limitations for the accurate identification of CNA regions when comparing tumor and normal samples.

Previously, the activity of heterologous enzymes displayed on a yeast cell surface was improved by increasing the copy number of the gene cassettes, for example by using the multi-copy genome integration method (δ-integration) [ 26].