These nanoparticle modifiers/conjugants include various antibodies and peptides which improve cell type and subcellular compartment targeting, while nucleic acids (and their mimics) have been demonstrated to modify subcellular retention of nanoconjugates.
As for other viral vector systems, AAV transcriptional as well as post-transcriptional strategies are under development to improve cell type specificity or to restrict transgene expression to a certain developmental stage.
We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors.
Thus, defining the in vivo binding profile for additional cell-specific regulators of cardiogenesis should vastly improve cell type-specific predictions, as we observed.
In addition, models integrating multiple data sources, including histone modification ChIP-Seq data, have been shown to successfully improve cell type-specific TF binding site prediction [ 36, 80].
Thus, AAVs may be used to improve cell-type enriched delivery of miRNAs.
The increase in striatal neurosphere number and diameter following mild inhibition of GSK-3 with BIO could be attributed to enhanced proliferation of both neural stem and progenitor cells or to improved cell survival of both cell types (Fig. 2C).
The SC-CO2-treated ECM material showed improved cell-material interactions for all cell types tested.
The technique improved cells' lifespan and function.
In a homogeneous population with only one cell type, any improved efficiency in production would correspond to an increased growth rate (Fig. 4a).
