Sentence examples for improve transformation efficiency from inspiring English sources
In order to improve transformation efficiency and to achieve higher GFP expression levels as well as stable transformants, a new series of cloning vectors for ATM transformation was constructed.
The kfoA and kfoC genes were sequentially cloned into plasmid pNZ8148 using L. lactis as intermediate host to improve transformation efficiency (Additional file 1).
Lysozyme was added to the bacterial cells at a final concentration of 10 μg/mL and the cells were then incubated at 37 °C for 30 min to improve transformation efficiency.
We modified the protocol by the following additional step: the yeast cells were incubated in reducing buffer (1 mM Tris, pH 7.5, 1 mM EDTA, 1 mM LiOAc, 10 mM DTT) at room temperature for 1 hour, followed by three washes of ice-cold sorbitol to improve transformation efficiency.
Therefore, proper DNA methylation could further improve transformation efficiency by blocking C. thermocellum restriction endonucleases.
This study showcased the difficulty of obtaining stable mutants in most Cyanothece strains and how to improve transformation efficiency by introducing linear, single-stranded, oligonucleotides carrying the desired mutations flanked by fragments homologous to a chromosomal insertion site.
This suggests that further optimization is required to improve transformation efficiencies and for more rapid identification and separation of mutants before they are visible in cultures.
To determine whether Dam methylated DNA improves transformation efficiency, unmethylated plasmid DNA was Dam methylated in vitro and transformed into C. thermocellum.
To explore the effects of overexpressing wild-type, mutant, and exogenous adhEs, we developed a new expression plasmid, pDGO144, that exhibited improved transformation efficiency and better gene expression than its predecessor, pDGO-66.
We then used a different Agrobacterium strain (GV3101) to verify that the improved transformation efficiency obtained with pABr1 was a genuine effect of the plasmid vector, rather than an indirect effect of the specific A. tumefaciens strain utilized for transformation.
Some reports have indicated that the mutants of B. subtilis [36], Thermosynechococcus elongatus [37], and Borrelia burgdorferi [38] deficient in restriction endonuclease activity exhibited improved transformation efficiency.
