Sentence examples for improve transformation from inspiring English sources

In order to improve transformation efficiency and to achieve higher GFP expression levels as well as stable transformants, a new series of cloning vectors for ATM transformation was constructed.

In this work, for the first time, we prepared chitosan/pDNA nanoparticles by electrospraying methods to improve transformation process.

The kfoA and kfoC genes were sequentially cloned into plasmid pNZ8148 using L. lactis as intermediate host to improve transformation efficiency (Additional file 1).

Lysozyme was added to the bacterial cells at a final concentration of 10 μg/mL and the cells were then incubated at 37 °C for 30 min to improve transformation efficiency.

We modified the protocol by the following additional step: the yeast cells were incubated in reducing buffer (1 mM Tris, pH 7.5, 1 mM EDTA, 1 mM LiOAc, 10 mM DTT) at room temperature for 1 hour, followed by three washes of ice-cold sorbitol to improve transformation efficiency.

Therefore, proper DNA methylation could further improve transformation efficiency by blocking C. thermocellum restriction endonucleases.

However, improved transformation efficiencies of approximately 105 transformants/μg DNA with the same system of host/vector were achieved by Dornan and Collins [ 10] by altering the conditions under which the bacteria were grown prior to electroporation, e.g. by incorporating threonine in the medium, while cells of the early-log phase were used.

To determine whether Dam methylated DNA improves transformation efficiency, unmethylated plasmid DNA was Dam methylated in vitro and transformed into C. thermocellum.

Lloret et al. (2010) showed an improved transformation of DCF in the presence of different mediators such as syringaldehyde or 1-hydroxybenzotriazole.

These precipitates can accommodate transformation strain, relieve constraint in adjacent austenite grains, and arrest cracks by extensive plastic deformation, thereby improving transformation ductility and shape memory effects.

These experiments showed equivalent or improved registration accuracy compared to other state-of-the-art (dis)similarity metrics and improved transformation consistency compared to pairwise mutual information.