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Overexpression of some DNA binding proteins could improve the transformation efficiency.
The results indicated that the following conditions could improve the transformation efficiency.
In order to further improve the transformation yield, the foreign electron traps of Pt and Pd nanoparticles were loaded on the film by the photoreduction approach.
In the present experimentation, a comprehensive and systematic study was performed to improve the transformation efficiency of kumquat, which was usually less than 4.1% in previous reports.
The results suggested that acid or erythromycin stress could improve the transformation efficiency of L. lactis through regulating gene expression of DNA binding proteins.
It can be concluded from this study that acidity is beneficial for conversion of asphaltenes but the hydrogenation active phase does not seem to be the main way to improve the transformation.
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Increasing the ratio of A. tumefaciens to target conidia to 1 × 104 improved the transformation frequency to 30 transformants per 1 × 104 conidia (Table 3, 48 h) on average; the single-copy integration of the T-DNA decreased to 30%, but the frequency of T-DNA integration into a single locus with the vector backbone typically increased to 55%.
We have proposed a simple but promising strategy for improving the transformation efficiency of L. lactis and other hard-transformed microorganisms.
Recent studies that identified several plant genes involved in Agrobacterium-mediated transformation, and their over-expression in currently transformable species, suggest that this approach holds great promise for improving the transformation of recalcitrant, but agronomically important, crops.
In addition, the single plasmid system improved the transformation efficiency compared to the dual plasmid system (from 3 × 102 to 2 × 104 colonies/μg DNA).
The new method improved the transformation efficiency of B. subtilis with linear integrative DNA nearly 700-fold compared with existing Bacillus transformation techniques.
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