Many efforts have been made to improve the expression of heterologous genes in the BEVS.
Therefore, more endeavors should be made to improve the expression level of this important enzyme.
In this study, we described a strategy to improve the expression efficiency using synthetic double promoter.
Under such circumstances, it might be much easier to improve the expression than the activity by several amino acids substitutions.
The results show that the two regulatory elements could also effectively improve the expression of PCV2 VLPs in Sf9 cells.
To improve the expression of shallow cloud, the results of large eddy simulation (LES) models have been utilized.
Careful optimization of cell culture conditions can drastically improve the expression level and homogeneity of the target protein.
Xu et al. demonstrated that vitamin D can inhibit Porphyromonas gingivalis-induced proinflammatory cytokine expression and improve the expression of anti-inflammatory cytokines in macrophages [53].
To further improve the expression of His6-PrS2-Bac7 (1–35), we atoempted to apply the SPP system for its production.
In order to evaluate the aforementioned hypothesis and to overcome the existing limitation, we attempted to improve the expression level of PHA synthase.
Therefore, to further improve the expression level of mBFP in plants, the codon usage might need to be optimized for plants in the future.
