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To improve the assay specificity, Juncker and his collaborators have developed a number of innovative platforms with improved performance [107 109].
Finally, using P3 and P5 with longer binding sites results in increased overall sensitivity of the reaction, showing that increasing ligation efficiency can improve the assay overall.
However, further data accumulation is still needed to improve the assay performance because the eROS assay provided false predictions for some compounds.
Studies to improve the assay performance in the field were performed, showing that assay activity could be preserved for up to 2 months.
Efforts to improve the assay sensitivity of analgesic trials could reduce the rate of falsely negative trials of efficacious medications and improve the efficiency of analgesic drug development.
This methodological extension to other prokaryotic genomes has required a partial primer re-design and protocol optimization, in order to improve the assay.
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It is evident that similar programs might benefit researchers seeking to improve the assays used in evaluating other antimicrobials (13).
Consequently, current investigations focus on analyses of the magnitude of temporal changes in serum cTn concentration in order to improve the assays' low positive predictive value for early diagnosis of AMI [ 27].
It was also established that the use of methanol as cosolvent significantly improved the assay sensitivity through the stabilization of the ternary complex.
Moreover, we greatly improved the assay sensitivity by the employment of AuNPs labelled with a high concentration of ECL signal probes carried by cysteamine.
By further improving the assay condition and format, we are able to detect fewer than ten targets in 1 h and to apply successfully this method for detection of Epstein Barr virus in human lymphoma specimens.
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